Table 1.
Strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Bacterial strains | ||
| E. coli DH5α | F− φ80dlacZΔM15Δ(lacZYA-argF)U169 endA1 recA1 hadR17 (rK− mK−) deoR thi-1 supE44 gyrA96 relA1 | Life Technologies |
| C. perfringens | ||
| JIR325 | Rifr Nalr derivative of strain 13 | 32 |
| JIR12233 | JIR325ΔrevRΩ erm(Q) | This study |
| Plasmids | ||
| pT7Blue-3 | Commercial cloning vector; Apr | Novagen |
| pJIR750 | E. coli-C. perfringens shuttle vector, carries pIP404 replication origin; Cmr | 5 |
| pJIR2715 | Suicide vector base for C. perfringens; Cmr Ermr | 6 |
| pJIR3374 | pJIR2715 (Asp718/BamHI)Ω JRP3520/JRP3521 (Asp718/BamHI, 1.8 kb) PCR product, Cmr Ermr | This study |
| pJIR3429 | pJIR3474 (XhoI/NotI)Ω JRP3718/JRP3719 (XhoI/NotI, 1.2 kb) PCR product, Cmr Ermr (revR suicide vector) | This study |
| pJIR3534 | pJIR750 (BamHI/Asp718)Ω JRP3954/JRP3966 (BamHI/Asp718, 1.2 kb, Cmr (revR+ complementation plasmid) | This study |