Table 2.
Relevant genes differentially expressed in the revR mutanta
| Category | Gene name | Locus tag | Fold ratiob | P valuec | Function |
|---|---|---|---|---|---|
| Upregulated genes | nanI | CPE0725 | 23.9 | 1.8E−05 | Sialidase (NanI) |
| CPE0185 | 16.9 | 8.5E−06 | Acylneuraminate lyase (sialic acid lyase) | ||
| colA | CPE0173 | 7.02 | 4.1E−04 | Collagenase (kappa-toxin) | |
| ccp | CPE0846 | 6.1 | 5.0E−03 | α-Clostripain | |
| Downregulated genes | nagH | CPE0191 | 0.245 | 0.031 | Hyaluronidase (mu-toxin) (NagH) |
| nanJ | CPE0553 | 0.127 | 3.3E−04 | Sialidase (NanJ) | |
| nagL | CPE1523 | 0.117 | 8.9E−05 | Hyaluronidase (mu-toxin) (NagL) | |
| CPE1423 | 0.253 | 8.8E−04 | Small acid-soluble spore protein C2 | ||
| CPE1753 | 0.081 | 2.39E−05 | Stage IV sporulation protein A | ||
| CPE2218 | 0.062 | 2.4E−06 | Probable spore coat protein | ||
| Other virulence genes | pfoA | CPE0163 | 1.16 | 0.72 | Perfringolysin O (theta-toxin) |
| plc | CPE0036 | 1.1 | 0.733 | Phospholipase C (alpha-toxin) |
a
RNA was extracted from 4-h exponential-phase cultures. Microarrays were hybridized with cDNA generated from four independent biological replicates with two dye swaps.
b
Expressed as a fraction of the wild-type expression level.
c
Determined using a modified t test algorithm.