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. 2011 Jun;79(6):2204–2214. doi: 10.1128/IAI.01382-10

Fig. 4.

Fig. 4.

CR3 and FcγRs restrict phagosomal escape of opsonized Schu S4. (A) BMMs from C57BL/6, SRA-KO, and MR-KO mice were infected with unopsonized Schu S4 and processed for phagosomal integrity assays at 20, 30, 45, 60, and 90 min p.i. (B) BMMs from C57BL/6, CD11b-KO, and SRA-KO mice were infected with serum-opsonized Schu S4 and processed for phagosomal integrity assays at 20, 30, 45, 60, and 90 min p.i. (C) BMMs from C57BL/6, FcRγ-KO, and gp91Phox-KO mice were infected with IgG-opsonized Schu S4 and processed for phagosomal integrity assays at 20, 30, 45, 60, and 90 min p.i. At least 100 bacteria were analyzed per condition. Values are expressed as the percentages of cytosolic bacteria and are the means ± standard deviations of three to four independent experiments. Asterisks denote statistically significant differences in phagosomal escape compared to C57BL/6 BMMs, according to a one-way analysis of variance followed by a Dunnett posttest (P < 0.05). In panels B and C, asterisks colors indicate the receptor knockout that yields statistically significant differences from the C57BL/6J control BMMs.