. 2011 Jun;79(6):2423–2429. doi: 10.1128/IAI.01169-10

Table 3.

Relative quantification of BAV1965 expression at 22°C and 37°C using the 2⁻^ΔΔCT method

Temp	C_T		ΔC_T^a	ΔΔC_T^b	Normalized BAV1965 amt^c
Temp	BAV1965	tyrB	ΔC_T^a	ΔΔC_T^b	Normalized BAV1965 amt^c
22°C	22.06	14.11
	21.88	14.15
	22.08	13.93
Avg	22.01 ± 0.11	14.06 ± 0.12	7.94 ± 0.21	0.00 ± 0.21	1.00 (0.87–1.16)
37°C	17.48	15.87
	17.92	15.88
	17.62	15.90
Avg	17.67 ± 0.23	15.88 ± 0.02	1.79 ± 0.22	−6.15 ± 0.22	71.18 (60.967–83.097)

ΔC_T = (avg C_T_,_BAV1965 − avg C_T_,_tyrB).

ΔΔC_T = (avg ΔC_T_{, target} − avg ΔC_T_{, 22°C}).

BAV1965 amount (2⁻^ΔΔCT) was normalized relative to the amount at 22°C. Values in parentheses indicate 95% confidence intervals.