Fig. 3.

Cxcl1 and Ccl2 expression in GAS-infected tissue. RNA was isolated from inflamed tissue at 24 and 72 h postinfection, and levels of Cxcl1 (A) or Ccl2 (B) transcripts were quantified in relation to that of Gapdh using qRT-PCR. Values reported are fold change compared to values for wild-type-infected tissue at 24 h. Graphs show means and SEM of results for 3 mice per group at each time, 2 to 3 technical replicates per mouse. (“*” indicates P < 0.05 by two-tailed, unpaired Student's t test; “**,” P < 0.01). Clear bars represent mice infected with wild-type bacteria; black bars represent mice infected with spyA mutant bacteria.