Fig. 4.

Expression of Mmp2 and Vim in infected tissue. RNA was isolated from inflamed tissue, and using qRT-PCR levels of Mmp2 (A) or Vim (B), transcripts were determined in relation to Gapdh transcripts and normalized to levels found in wild-type-infected tissue at 24 h. Graphs show means and SEM of 3 mice per group (2 to 3 replicates per mouse) at each time. *, P < 0.05; **, P < 0.01. A two-tailed, unpaired Student's t test was used to compare the wild type to the mutant, and ANOVA followed by Tukey's posttest was used to compare across time points. White bars represent wild-type bacteria, and black bars represent spyA mutant bacteria.