Fig. 5.

Internalization of GAS into HeLa cells. Wild-type and spyA mutant bacteria were incubated with HeLa cells for 3 h, at which point associated bacteria were quantified, or cells were treated with antibiotics for 1 h. No difference was detected in associated bacteria at 3 h. The graph shows the percentage of associated bacteria that remain viable after antibiotic treatment. “**” indicates P < 0.01 using a two-tailed, paired Student t test. Bars show means and standard errors of results from 7 independent experiments.