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. 2011 Mar 7;20(5):834–848. doi: 10.1002/pro.609

Figure 4.

Figure 4

GSTpi binding to active JNK1/ATF2 complexes. Binding experiments in which 3.5 μM ATF2 and 3.5 μM active JNK1 were added to the Ni-NTA resin, and the V105/V114 GSTpi was added in increasing concentrations (from 0.5 to 10 μM). A, Silver staining of the SDS-PAGE gel of the eluted samples of all initial GSTpi concentrations tested. B, Western blot analysis using antibodies against GSTpi of the eluted samples described in panel A.