Figure 5.

GSTpi interaction with ATF2 in the absence of JNKs. Resin equilibrated with 3.5 μM ATF2 was mixed with initial 7 μM V105/V114 GSTpi, as was described in the Experimental Procedures. A, The amount of bound GSTpi in the eluted fractions was monitored by Western blotting analysis using antibodies against GSTpi. B, Plots of the densitometry analysis from panel A (no JNK) as well as the results from binding experiments when inactive JNKs were present [from Fig. 3(B)]. The total nmol of eluted GSTpi were calculated as was described for Figure 3.