Figure 8.

Effect of S-methyl and S-hexylglutathione on the interaction between GSTpi and ATF2. Binding experiments between GSTpi and ATF2 were carried out either in the regular buffer or in the buffer containing 2.5 mM S-methylglutathione (S-Me-G) or 2.5 mM S-hexylglutathione (S-Hex-G) (as described in the Experimental Procedures). A, Western blot analysis using antibodies against GSTpi of the imidazole eluates. Silver staining was also performed on the gel to demonstrate the corresponding amounts of ATF2 that were found in the imidazole elutions. B, Plot of the densitometry analysis of the Western blot in panel A to show the total eluted GSTpi amounts (nmol).