Figure 2.

Overall tertiary structure of WbgU/NAD(H)/UDP-GlcNAc complex. N-terminal domain is a modified Rossmann type fold with the cofactor NAD(H) sitting on and parallel to the carboxy edge of the β sheet. The C-terminal domain binds the substrate UDP-GlcNAc. β strands are numbered from the N- to the C- terminal. Dashed grey lines represent the six-residue loop from His287-Ile293 that was not modeled due to poorly defined electron density. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]