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. Author manuscript; available in PMC: 2012 Jun 14.
Published in final edited form as: Biochemistry. 2011 May 18;50(23):5301–5313. doi: 10.1021/bi200329t

Table 2.

Mutational analysis of MjCobY dimerization interfaces.

Protein tested Functional
in vivo?		 Doubling
time
(min)		 Monomer:Dimer:
Aggregate Ratio		 Specific Activity
(pmol/min/µg protein)
Mixture Monomer Dimer Aggregate
MjCobYWT Yes 79 ± 3 59: 41: 0 103 ± 5a ND ND NA
MjCobYL38D, K41D Yes 78 ± 1 52 : 26 : 22 ND 124 ± 6 119 ± 2 85 ± 7
MjCobYL38K, K41D Yes 79 ± 1 53 : 25 : 22 ND 125 ± 2 120 ± 10 78 ± 3
MjCobYI144D, F146D, Y163R Yes 83 ± 4 77 : 23 : 0 ND 156 ± 23 49 ± 10 NA
a

MjCobYWT specific activity of a mixture of monomers and dimers. Doubling time was determined during growth in minimal medium containing glycerol (30 mM) + CNCby (15 nM).

ND, not determined; NA, not applicable.