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. Author manuscript; available in PMC: 2011 Jun 29.
Published in final edited form as: Cell Biol Int. 2009 Dec 16;34(1):41–47. doi: 10.1042/CBI20090016

Figure 4. The CC domain of ERK2 is required for pERK1/2 translocation to focal adhesions.

Figure 4

(A) HeLa cells were transfected with pcDNA3 (a and b) or FLAG–ERK2(del CC) (c and d) for 24 h, serum-starved for 6 h, and then stimulated with EGF as indicated. Cells were fixed with 4% formaldehyde and stained with an anti-pERK1/2 antibody. (B) HeLa cells were transfected with pcDNA3 (a, b and c) or FLAG–ERK2(del CC) (d, e and f) for 24 h, serum-starved for 6 h, and then stimulated with EGF for 10 min. Cells were fixed with 4% formaldehyde and stained with an anti-pERK1/2 antibody (a and d) or anti-GIT1 antibody (b and e). Panels c and f are the merged images of a and b, and d and e respectively. These results were reproducibly obtained in three independent experiments.