FIGURE 2.
Western blot analysis for the expression of PXR or CYP3A4 protein in nontreated cells. Total cell lysates (50 µg) were separated on a 10% SDS-PAGE gel and immunoblotted with polyclonal anti-PXR antibodies against the conserved epitope (A-20) or the n-terminal (N-19), and detected using the enhanced chemiluminiscent substrate.