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. Author manuscript; available in PMC: 2011 Jun 29.
Published in final edited form as: Cancer. 2007 Mar 1;109(5):957–965. doi: 10.1002/cncr.22479

FIGURE 8.

FIGURE 8

(A) COL and OS187 cells were cultured with 150 µM etoposide for 24 hours and then examined by flow cytometry, using FITC-annexin V and 7-amino-actinomycin D (7AAD). Viable cells do not stain with either reagent. Staining with 7-AAD alone indicates necrotic cells. Staining with Annexin V identifies apoptotic cells and 7AAD staining separates early apoptotic (7AAD−) from late apoptotic (7AAD+) cells. (B) Viable COL cells remaining after exposure to etoposide, or ketoconazole and in combination for 24 hours (−) and 48 hours (−). (Keto+ represents simultaneous addition of both agents; Keto* represents 24 hours pretreatment with ketoconazole before the addition of etoposide.)