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. Author manuscript; available in PMC: 2011 Jun 29.
Published in final edited form as: Br J Haematol. 2008 Nov 1;144(1):120–126. doi: 10.1111/j.1365-2141.2008.07416.x

Figure 4.

Figure 4

Pyrene actin polymerization assays show that GBD-VCA I294T display Cdc42-independent activation. Pyrene fluorescence at 407 nm (excitation wavelength 365 nm) was monitored over time, as described in Materials and Methods. Control : 4 μM actin (6% pyrene), 10 nM bovine Arp2/3 complex. 500 nM VCA, 500nM GBD-VCA or 500 nM GBD-VCA I294T were added as indicated.