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. Author manuscript; available in PMC: 2011 Jun 29.
Published in final edited form as: Science. 2009 Dec 10;327(5964):466–469. doi: 10.1126/science.1179663

Fig. 4.

Fig. 4

Mycobacterial ESAT-6 is sufficient to induce mmp9 in epithelial cells independent of Myd88 and TNF signaling. (A-D) Relative mmp9 expression analyzed by qRT-PCR of (A) con or pu.1 morphant embryos 3 dpi with 84 WT or 126 ΔRD1 (represents one biological replicate), (B) 34 hpf embryos 4 hours post-injection with WT or ΔRD1 bacterial supernatant. Bars represent means ± SEM of three biological replicates. (C) 34 hpf con embryos 4 hours post-injection with 4.8×10−17 moles of purified ESAT-6 or CFP-10, or 4.9×10−17 moles of ESAT-6 plus 5.0×10−17 moles of CFP-10. Bars represent means (± SEM) of five biological replicates. (D) 34 hpf con embryos, myd88 morphants, or tr1 morphants 4 hours post-injection with 5.7×10−17 moles of purified ESAT-6. Bars represent means ± SEM of four biological replicates (pu.1 morphant), or three biological replicates (myd88 and tr1 morphants).