Figure 1.

Targeted deletion and expression of math5. (A) math5 genomic structure, restriction enzyme map, and targeting strategy. The DNA fragment containing the math5 ORF region was replaced with GFP or lacZ in the targeting vector. Neo, PGK-neo cassette; TK, MC1-TK cassette. (B) Southern genotyping of a typical litter from a heterozygous cross. The 5′ probe detected 5.1-kb wild-type and 2.7-kb mutant EcoRV fragments.