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. 2001 Jan 1;15(1):36–41. doi: 10.1101/gad.852101

Figure 4.

Figure 4

In vitro spliceosomes and commitment complex formation. (A) Extracts from DLY23 strain prepared in the absence (lanes 1–4 and 9–12) or presence (lanes 5–8 and 13–16) of doxycycline. For commitment complex formation, the extract was U2snRNA inactivated (lanes 9–11 and 13–15) or ATP depleted before incubation with the RNA (lanes 12,16). (B) Complex gel analysis of spliceosome assembly reactions from sub2-201 extracts. Heat treatment (even lanes) was for 20 min at 37°C. Mock treatment (odd lanes) was on ice. M: CC2 marker lane (U2snRNA inactivated extract). The ratios CC2/(pre-)spliceosome are as follows: lane 1: 0.87; lane 2: 1.42; lane 3: 0.33; lane 4: 0.69; lane 5: 0.27; lane 6: 0.90. The increase in this ratio on heat treatment is as follow: 3 min: 1.62; 15 min: 2.11; 40 min: 3.33.