Figure 1.

Targeted deletion of Sox10 and expression of the Sox10^lacZ allele in mice. (a) Schematic representation of the targeting strategy employed to disrupt the Sox10 gene. Shown are the targeting vector (top), the wild-type Sox10 locus (middle) and the mutated locus after homologous recombination (bottom). Noncoding and coding exons are represented by black or open boxes, respectively. Restriction sites for SpeI (P) and NcoI (N) as well as the location of the 5′ probe are indicated. (b) Southern blot analysis of DNA from adult heterozygous (+/−) and wild-type (+/+) mice after digestion with NcoI/SpeI. The expected size of the fragments detected by the probe used for hybridization are shown (a). (c) RT–PCR analysis of wild-type (+/+), heterozygous (+/−) and homozygous (−/−) embryos at E12.5 using primers specific for the coding region of Sox10. Results from 20, 23, and 26 cycles are shown for each genotype. Sox10^lacZ/+ (d–f) and Sox10^lacZ/Sox10^lacZ (g–i) embryos at E8.5 (d,g), E10.5 (e,h), and E12.5 (f,i) after X-gal staining.