Figure 4.

Sox10 controls peripheral glial cell development. (a–f) Immunohistological analysis of wild-type (a,b), Sox10^Dom/Sox10^Dom (c,d), and ErbB3^−/−(e,f) embryos at E11.5 on lumbar axial levels, using antibodies against B-FABP (red) and TuJ-1(green) to visualize peripheral glial cells and neuronal cells, respectively. B-FABP antibody signals (a,c,e) and an overlay of B-FABP and TuJ-1 antibody signals (b,d,f) are shown. The arrowhead in d points towards the abnormally shaped dorsal root entry zones in Sox10^Dom/Sox10^Dom mutants; the arrows point towards B-FABP-positive Schwann cell precursors that line spinal nerves in control and ErbB3^−/− mice. (g–l) In situ hybridization of wild-type (g,h), Sox10^Dom/Sox10^Dom (i,j), and ErbB3^−/− (k,l) embryos at E11.5 with probes specific for Sox10 (g,i,k) or Notch-1 (h,j,l). Sections on lumbar axial levels are shown. Bars (a–f) 100 μm; (g–l) 150 μm.