Figure 5.

Appearance of dorsal root ganglia in homozygous Sox10 mutant animals. Histological and immunohistological analyses of dorsal root ganglia and spinal nerves in Sox10^lacZ/+ (a,b), wild-type (c–e), Sox10^lacZ/Sox10^lacZ (f,g), and Sox10^Dom/Sox10^Dom (h–j) animals at E11.5 (a,b,f,g) and E12.5 (c–e,h–j). (a,f) Immunohistological analysis using antibodies against B-FABP (red) and β-galactosidase (green). Note that B-FABP and β-galactosidase from the Sox10–lacZ reporter are coexpressed (yellow) in satellite glia of dorsal root ganglia of control mice. B-FABP is not expressed in Sox10^lacZ/Sox10^lacZ mice, although β-galactosidase-positive cells persist. (b,g). High-magnification of ventral roots stained with antibodies against peripherin (red) and β-galactosidase (green). Arrowheads point towards cells that express β-galactosidase; arrows point towards neuronal cells expressing peripherin. Note that the two proteins are coexpressed in neither control nor homozygous mutant mice, indicating that the β-galactosidase-positive precursor cells have not adopted a neuronal fate in Sox10^lacZ/Sox10^lacZmice. (c,h) Immunohistological analysis with antibodies against TuJ-1 (green) and B-FABP (red), which visualize neuronal and glial cells, respectively. Histological appearance of dorsal root ganglia (d,i) and intercostal nerves (e,j) at E12.5. Note the marked reduction in size of the ganglion in the mutant mice. In control mice, nuclei associated with the intercostal nerve are abundant (arrows in e), which are not observed in the Sox10 mutant. Note also the abnormal dorsal root entry zones in homozygous mutants, which are indicated by arrowheads in f, h, and i. Bars (a,f) 80 μm; (b,g) 15 μm; (c,d,h,i) 150 μm; (e,j) 50 μm.