Fig. 2.

The adapted virus contains 7 mutations relative to the wild type. (A) A cartoon map showing the J6/JFH-1 construct, where J6 is core-NS2 and JFH-1 is NS3-NS5B. Indicated are the locations of the 7 mutations found in the adapted virus. (B) The All-7 virus replicates in cells similarly to the adapted virus. NS3-4A protease activity levels for wild-type virus, adapted virus, All-7 virus, and a mock infection control are indicated. Error bars indicate standard deviations of data from 3 assays.