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Intracellular RNA replication for wild-type HCV, Min3, and the HCV single mutants is similar following RNA transfection under conditions of limited viral spread. Intracellular HCV RNA levels for the wild type, Min3, core K78E, NS2 W879R, and NS4B V1761L are indicated. The intracellular RNA levels were normalized relative to the wild-type intracellular RNA levels at 4 h following RNA transfection. Error bars indicate standard deviations of data from 2 assays.
