Table 1.

Effects of reversion of single mutations on infectivity posttransfection and postinfection

Virus descriptiona	Protein	Result at 72 h postinfection (MOI = 0.5)
		NS3 accumulation (% of All-7)b	Virus titer (TCID50/ml)c
Wt		15.3 ± 10.6**	1.30 × 102
All-7		100.0 ± 11.6	1.40 × 104
Rev K78E	Core	30.8 ± 9.1**	2.00 × 103
Rev S401N	E2	104.6 ± 13.5	2.50 × 104
Rev W879R	NS2	46.2 ± 8.9**	2.00 × 103
Rev Y904H	NS2	110.4 ± 22.2	2.50 × 104
Rev V1761L	NS4B	67.5 ± 18.6*	4.60 × 103
Rev D2173G	NS5A	132.4 ± 17.9	1.40 × 104
Rev V2417A	NS5A	119.3 ± 19.9	1.40 × 104

^aWt, wild type. “Rev” indicates reversion of the mutation to the wild-type residue.

^bStandard deviations of data from three independent experiments are given (two-tailed t test relative to data for All-7 clone (*, P < 0.05; **, P < 0.005).

^cVirus titers calculated from one of three representative experiments.