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Comparison of ISG15 and IRF7 transcriptional activation in PKR−/− and IRF3−/− MEFs. PKR- or IRF3-deficient MEFs were infected with UK or transfected with poly(I:C). At 6 hpi, RNA was prepared, and levels of ISG15 and IRF7 transcripts were quantitated by qRT-PCR. Data are presented as fold induction over levels of mock-infected controls, and error bars are from triplicate measurements. Results are representative of two independent experiments with similar results.
