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DUB activity assay during FMDV infection. IBRS-2 cells cultured in 60-mm dishes were transfected with HA-tagged ubiquitin and infected with FMDV at different MOIs at 24 h posttransfection. Cells were lysed at 12 h postinfection and assayed for anti-HA and anti-beta-actin staining by Western blotting. The asterisk indicates a nonspecific band.
