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. 2011 Apr;85(8):3767–3779. doi: 10.1128/JVI.02438-10

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Copyright © 2011, American Society for Microbiology

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Fig. 2. — Replication of the HSIV-vif chimera. (A) Schematic diagram comparing the genomic structures of HIV-1_NL4-3, HSIV-vif, and SIVmne027. (B) Replication of HIV-1_NL4-3 and HSIV-vif in anti-CD3/anti-CD28-costimulated human PBMC. (C) Replication of HSIV-vif in comparison to SIVmne027 and HIV-1_NL4-3 (NL4-3) in anti-CD3/anti-CD28-costimulated Pt PBMC. Duplicate infections were carried out at an MOI of 0.01. Replication was monitored by determining the amount of infectious virus produced per ml at 3- to 4-day intervals postinfection using TZM-bl reporter cells. Infections of two different macaque donors are shown and were performed using independently derived stocks of virus.