Fig. 3.

Generation of UL71 stop mutant TBstop71. (A) Position of the introduced stop codon and the frameshift mutation at the 5′ prime of UL71. In TBrev71-BAC, the stop and frameshift mutation was restored to the original UL71 sequence with a silent mutation (indicated by the gray box). (B) Restriction fragment length polymorphism analysis of BAC-DNAs of TB40-BAC4 (wild type), TBstop71-BAC, and TBrev71-BAC with restriction enzymes EcoRI and HindIII. The three BAC-DNAs were used to reconstitute the respective viruses. Lanes M, molecular marker (1-kb DNA ladder; Invitrogen).