Fig. 7.

Three-dimensional reconstruction of membranes and viral capsids from tomography of TBstop71 virus-infected HFFs at 5 days postinfection. (A) Single slice from the three-dimensional tomogram with a large vesicle in which multiple budding processes of TBstop71 virus particles are observed. (B) Membranes used as budding sites as well as viral capsids were reconstructed and superimposed on the original micrograph. Membranes are depicted in light blue; viral capsids are indicated in dark blue. (C) Reconstruction of the budding processes only. Bars, 200 nm. Note that areas with high mass density, such as membranes, occur bright in the dark-field STEM tomograms. See Movie S1 at http://www.uniklinik-ulm.de/struther/institute/virologie/home/forschung/publikationen/suppl.html.