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Subcellular localization of MVB marker CD63 and viral pp28 in wild-type and TBstop71 virus-infected HFFs (MOI = 0.1 to 0.5). Cells were fixed with 4% PFA in PBS at 7 days postinfection. CD63 and pp28 were detected by the respective MAbs and visualized by isotype-specific secondary antibodies conjugated to Alexa Fluor 488 (for pp28) and 555 (for CD63). Cell nuclei were visualized by using DAPI.
