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. 2011 Apr;85(8):3733–3745. doi: 10.1128/JVI.02546-10

Fig. 7.

Fig. 7.

The endogenous bovine TRIM56 restricts BVDV replication in MDBK cells. (A) Real-time RT-PCR analysis of boTRIM56 mRNA expression in parental MDBK cells and two MDBK cell clones stably transfected with a boTRIM56 shRNA (T56i#2 and T56i#3). (B) Effect of the stable knockdown of boTRIM56 in MDBK cells on viral induction of the type I IFN response. Parental MDBK and MDBK-T56i #2 and -T56i#3 cells were mock treated, infected with SeV, or transfected with poly(I:C) (pIC) or poly(dA:dT) (pdAdT). Cells were lysed for immunoblot analysis of bovine ISG15, MxA, and actin expression. Note that MDBK cells did not respond to transfected poly(dA:dT) to induce ISG15 and MxA expression. (C) Replication of the BVD39 replicon in MDBK and MDBK-T56i #2 and -T56i#3 cells. Data shown are representative of two independent experiments.