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. 2011 Apr;85(8):3733–3745. doi: 10.1128/JVI.02546-10

Fig. 9.

Fig. 9.

TRIM56 does not promote the degradation of BVDV NS proteins. 293FT cells grown in 6-well plates were mock transfected or cotransfected with 0.25 μg of the indicated Myc- or Myc-6×His-tagged ncpBVDV NS protein construct and 1.5 μg of the V5-tagged TRIM56 (WT or the CC21/24AA mutant [AA]) vector. Cells were lysed and subjected to immunoblot analysis of the expression of BVDV NS proteins (using anti-Myc antibody) and TRIM56 (using anti-V5 antibody). Asterisks indicate nonspecific bands.