Table 1.

Primers used for mutagenesis of human TRIM56

Mutation	Primer	Primer sequence
A (ΔRING)	del2-71F	5′-CCTTCGCGGATCCATGTCCTTCAAGACCAACT-3′
	del2-71R	5′-AGTTGGTCTTGAAGGACATGGATCCGCGAAGG-3′
B (ΔB box)	del161-205F	5′-GTGGTATGATGAGGAGGCTGAAGCTGTGCGTG-3′
	del161-205R	5′-CACGCACAGCTTCAGCCTCCTCATCATACCAC-3′
C (Δcoiled coil)	del211-363F	5′-GCTGAAGCTGTGCGTCACCTTCTTCGGCTG-3′
	del211-363R	5′-CAGCCGAAGAAGGTGACGCACAGCTTCAGC-3′
D (Δ369-742)	del369-742F	5′-CACCTTCTTCGGCTGGGGACCATCCACATC-3′
	del369-742R	5′-GATGTGGATGGTCCCCAGCCGAAGAAGGTG-3′
E (Δ77-742)	del77-742F	5′-CTCCTTCAAGACCAACGGGACCATCCACATCT-3′
	del77-742R	5′-AGATGTGGATGGTCCCGTTGGTCTTGAAGGAG-3′
F (ΔN363)	del2-363F	5′-CTTCGCGGATCCATGCACCTTCTTCGGCTG-3′
	del2-363R	5′-CAGCCGAAGAAGGTGCATGGATCCGCGAAG-3′
G (ΔRING + B box)	del2-205F	5′-CTTCGCGGATCCATGGCTGAAGCTGTGCGT-3′
	del2-205R	5′-ACGCACAGCTTCAGCCATGGATCCGCGAAG-3′
H (Δ211-284)	del211-284F	5′-GCTGAAGCTGTGCGTGAAGAAGCTGCTCGG-3′
	del211-284R	5′-CCGAGCAGCTTCTTCACGCACAGCTTCAGC-3′
I (Δ290-353)	del290-353F	5′-GAAGAAGCTGCTCGGCTCCATCCTGGGCTC-3′
	del290-353R	5′-GAGCCCAGGATGGAGCCGAGCAGCTTCTTC-3′
J (Δ355-433)	del355-433F	5′-CCACAGCTGGAGCTCACCTTGGAGGAGGAC-3′
	del355-433R	5′-GTCCTCCTCCAAGGTGAGCTCCAGCTGTGG-3′
K (Δ431-519)	del431-519F	5′-ACAACCCGAGAAGAGGAGATCCTGGTGGCG-3′
	del431-519R	5′-CGCCACCAGGATCTCCTCTTCTCGGGTTGT-3′
L (Δ515-610)	del515-610F	5′-GATCACCGGGCTCTGTGTGGAGGTGTACAATA-3′
	del515-610R	5′-TATTGTACACCTCCACACAGAGCCCGGTGATC-3′
M (Δ621-695)	del621-695F	5′-AATATGGAAGGCAGCCTGCTTCGAGAAGTCAACAAG-3′
	del621-695R	5′-CTTGTTGACTTCTCGAAGCAGGCTGCCTTCCATATT-3′
N (Δ693-750)	del693-750F	5′-GATAAGAAGGGCTACATCCGTTCTCCGGACAGTATC-3′
	del693-750R	5′-GATACTGTCCGGAGAACGGATGTAGCCCTTCTTATC-3′
C24A	TM56 C24A+	5′-ACTTCCTGGCCTGTAAAATCGCCCTGGAGCAGCTGCG-3′
	TM56 C24A−	5′-CGCAGCTGCTCCAGGGCGATTTTACAGGCCAGGAAGT-3′
CC21/24AA	TM56 CC21/24AA+	5′-AGCGACTTCCTGGCCGCTAAAATCGCCCTGGAGCAGCTGCG-3′
	TM56 CC21/24AA−	5′-CGCAGCTGCTCCAGGGCGATTTTAGCGGCCAGGAAGTCGCT-3′