Fig. 3.

Southern blotting to determine the number of gene copies of the A3Z3 gene in the porcine genome. (A) Hybridization site of the probe within the A3 gene locus as well as the nucleotide positions of the restriction sites used for digestion of the genomic DNA. (B) Genomic DNA from different species was digested with the restriction enzymes EcoRI (lanes 1 to 5) and BglII (lanes 6 to 10). The membrane was hybridized with the radiolabeled C-terminal porcine A3Z3 probe. The λ-HindIII-marker served as a size standard. Lanes 1 and 10, human; lanes 2 and 9, camel; lanes 3 and 8, pig; lanes 4 and 7, sheep; lanes 5 and 6, cow.