Fig. 5.

Coimmunoprecipitation of MDA5 and the HPIV-2 V protein. A549 cells transiently expressing both FLAG-tagged MDA5 and Myc-tagged WT or mutant HPIV-2 V protein bearing the C-terminal mutations indicated were lysed at 48 h posttransfection and subjected to immunoprecipitation (IP) with antibodies specific to the Myc tag. Myc-immunoprecipitated proteins or total cell lysates were separated by SDS-PAGE and processed for immunoblotting with antiserum against MDA5 or HPIV-2 V protein, as indicated. Lysates from cells transfected with empty vector or with vector expressing Myc-tagged GST protein served as negative controls. Results are representative of several independent experiments.