Fig. 11.

Bax contributes to, but is not required for, Smac release in reovirus-infected neurons in vivo. Coronal sections from WT (n = 4) and Bax^−/− (n = 4) mice infected with 100 PFU T3D at 9 dpi were analyzed by immunofluorescence for Smac release and were compared to those from mock-infected mice (n = 3 each). (A) Representative image of Smac (green), reovirus σ3 (red), and Hoechst (blue) staining in the thalamus of mock-infected Bax^−/− and T3D-infected WT and Bax^−/− mice. (B and C) Color histograms from one representative imaging experiment were obtained for individual cells (n = 51 per group) for mock-infected and T3D-infected WT and Bax^−/− mice to analyze the intensities of Smac staining. (B) The percentage of pixels between 10 and 255 was determined per cell, and the average is shown per group. (C) The average standard deviation of Smac intensities per cell is shown per group. ***, P < 0.0001.