Fig. 3.

Bax is important for reovirus growth in the brain. WT and Bax^−/− mice were injected with 100 PFU T3A i.c. and sacrificed at 8 dpi. (A) Coronal sections were stained by immunofluorescence for reovirus antigen σ3 (green) and Hoechst (blue) in the cingulate cortex (Cing), hippocampus (Hippo), and thalamus (Thal) of mock- or T3A-infected WT and Bax^−/− mice (100×). An asterisk indicates the antigen-positive ependymal cells lining the lateral ventricle in the Bax^−/− brain. (B) The percentage of reovirus-infected neurons was determined per high-power field (HPF) (400×) in the cingulate (n = 14 for WT [Bax⁺], and n = 10 for Bax^−/− [Bax−]), hippocampus (n = 12 for WT, and n = 10 for Bax^−/−), and thalamus (n = 14 for WT, and n = 9 for Bax^−/−). Lines between bars indicate comparisons. ***, P < 0.0001. (C) Viral titers from WT (closed circles) and Bax^−/− (open circles) brains of mice infected with 100 PFU T3A at 8 dpi. Each circle represents an average viral titer from one mouse brain.