Fig. 5.

Phylogenetic analysis of the complete sequences of the Candiru complex. All Candiru sequences were aligned using the CLUSTAL algorithm at the amino acid level with additional manual editing to ensure the highest possible quality of alignment. NJ analysis at the nucleotide level was performed. The statistical significance of tree topology was evaluated by bootstrap resampling of the sequences 1,000 times. Phylogenetics analyses were performed using MEGA software (33). Three clades were identified: clade A (green shading), clade B (blue), and clade C (red).