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. 2011 Apr;85(8):3950–3958. doi: 10.1128/JVI.01787-10

Fig. 2.

Fig. 2.

Eviction of core protein VII is not dependent on active transcription. (A) A549 cells were infected with hdAd-lacZ (MOI = 100 infectious particles/cell), and β-Gal activity was determined at the indicated time points (expressed in relative light units [RLU]). Representative data from two experiments are displayed; error bars represent the ranges of values obtained from two replicate samples. (B) A549 cells were pretreated with 500 μM DRB (or DMSO vehicle) for 1 h and infected with hdAd-lacZ (100 infectious particles/cell), and β-Gal activity was determined at the indicated time points (expressed in relative light units [RLU]). (C) A549 cells were pretreated for 1 h with 500 μM DRB or vehicle (DMSO) and infected with hdAd-lacZ (250 infectious particles/cell), and at the indicated time points, whole-cell lysates were prepared and subjected to immunoblotting with anti-FLAG to detect core VII or anti-α-tubulin. (D) A549 cells were infected with a hdAd vector lacking a transgene (hdAdΔ28; 2,500 total particles/cell), and at the indicated time points, nuclei were isolated, and protein extracts were prepared and subjected to immunoblotting with anti-core VII or anti-H3 as a loading control.

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