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Analysis of the SC-2 sF protein aggregation state by velocity sucrose gradient centrifugation. Freshly prepared and purified SC-2 was incubated at 4°C or 50°C for 1 h before loading on top of a linear 25%-to-55% sucrose gradient for ultracentrifugation in an SW41 rotor at 41,000 rpm for 20 h. The protein in each fraction was TCA precipitated, separated by SDS-PAGE, and detected by Western blotting with 5His MAb. The top fraction of the gradient of the gradient is indicated.
