Fig. 6.

Sucrose velocity gradients and electron micrographs of the SC-2 sF protein following dialysis with six different buffers. A 1-ml aliquot of freshly prepared and purified SC-2 in 50 mM phosphate (PO₄) buffer containing 500 mM NaCl and 250 mM imidazole was dialyzed against six different buffers for 18 h at 4°C before sucrose velocity gradient and EM analyses. The buffers and the net salt molarity are listed above each sucrose gradient panel. Proteins were detected by Western blotting as described in the legend to Fig. 5.