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. 2001 Jan 15;15(2):128–133. doi: 10.1101/gad.863801

Figure 1.

Figure 1

RPN11 is required for normal resistance to UV radiation (A). Isolation of temperature-sensitive mutants. Tenfold serial dilutions of cultures of YJR124-0 (RPN11), YJR124-8 (rpn11-8), YJR124-14 (rpn11-14), YJR124-22 (rpn11-22), YJR124-25 (rpn11-25) were spotted onto YPAD plates and incubated at 23°C, 30°C, or 37°C for 3 d. The amino acid substitutions in each allele are indicated below. (B) UV (left) and MMS (right) sensitivity of RPN11 mutants. For UV sensitivity studies, cells (YJR124) were grown in YPAD at 30°C until mid-log, and then appropriate dilutions were spread onto pre-warmed YPAD plates in triplicate. The plates were treated with the doses of UV radiation indicated in the figure and then incubated at 30°C in the dark. Sensitivity to MMS was measured on plates containing 0.01% and 0.03% MMS incubated at 30°C for 2 and 4 d, respectively. (C) Expression of RNR3 and HUG1 in RPN11 mutants. Cells were treated as described in Fig. 2A. RNR3 and HUG1 mRNA was detected by Northern blotting. ScR1 served as a loading control.