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. 2001 Jan 15;15(2):128–133. doi: 10.1101/gad.863801

Figure 3.

Figure 3

RPN11 functions downstream of RAS2. (A,B) The expression of stress-induced genes is not affected by RPN11 mutations. Wild-type (JR124-0) and mutant strains were grown in YPAD at 30°C to an OD of 0.4 and then treated with 1 mM hydrogen peroxide (H2O2, A) or 1 M NaCl (B) for 90 min. RNA levels were measured by Northern blotting. (C) MMS-induced expression of HIS4 in strains overexpressing Ras2val19. Wild-type and mutant strains containing a high-copy number plasmid expressing the activated form of RAS2 (Whistler and Rine 1997) were grown in selective media and treated with 0.1% MMS for 1 h (+). RNA was analyzed as described in Fig. 2B.