Fig. 1.

Hantavirus infection of endothelial cells. (A) Hantavirus replication in HUVECs and Vero E6 cells. HUVECS and Vero E6 cells were infected with PHV and TULV at a multiplicity of infection (MOI) of 1. Viral titers were analyzed 1 to 3 days postinfection by infectious focus assay as previously described (7). (B) HUVECs were either infected with PHV or TULV (MOI of 1) or mock infected. Cells were methanol fixed at 1 to 3 days postinfection, and infected cells were detected by immunostaining using an antinucleocapsid antibody as previously described (7).