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. 2011 May;85(10):4910–4926. doi: 10.1128/JVI.00011-11

Fig. 12.

Fig. 12.

Amounts of cell culture supernatant virus produced by F-BAC gB−/gD− and characterization of these particles for gE/gI and gH. HaCaT cells were infected with 10 PFU/cell of F-BAC gB−/gD−, F-BAC gD-kan, or F-BAC and radiolabeled from 6 to 13 h postinfection (p.i.); then, cell culture supernatants were harvested at 24 to 28 h p.i. Virus particles were partially purified by centrifugation through dextran-10 cushions, and then particles were resuspended in NP-40–DOC buffer. (A) Equal amounts of the total virus preparations were subjected to electrophoresis following addition of 2% sodium dodecyl sulfate and 2% β-mercaptoethanol and boiling. (B) gH was immunoprecipitated from NP-40–DOC extracts of the virus particles using MAbs LP11, 52S, and 53S. (C) gE/gI was immunoprecipitated from NP-40–DOC extracts of virus particles using MAbs 3114 and II-481. For panels B and C, note that 4-fold more of the supernatant was immunoprecipitated from F-BAC gB−/gD−-infected cells than from F-BAC- and F-BAC gD-kan-infected cells and 2-fold more immunoprecipitated material was loaded (totaling 8-fold more material). Molecular mass markers (in kilodaltons) are shown on the left side of the gels. The relative amounts of gE, gI, and gH are shown below panels B and C.