Fig. 2.

Characterization of gB and gD expressed in noncomplementing and complementing cells. Vero cells (which do not express HSV glycoproteins), VB38 cells (which express gB) (columns labeled “38”), and VD60/gB47 cells (which express gB and gD) (columns labeled “47”) were infected with wild-type (w.t.) F-BAC or F-BAC gB⁻/gD⁻. After 5 to 6 h of infection, the cells were radiolabeled with [³⁵S]methionine-cysteine for 2 to 3 h; then, cell extracts were made using NP-40–DOC buffer. gB or gD was immunoprecipitated from cell extracts using rabbit polyclonal antibodies (R68, specific for gB, and R45, specific for gD), and proteins were subjected to electrophoresis in 6% (gB) or 10% (gD) polyacrylamide gels. Molecular mass markers (in kilodaltons) are shown on the left side of the gels.