Table 1.
Numbers of unenveloped, partially enveloped, and fully enveloped particles in Vero and VD60/gB47 cells infected with F-BAC gB−/gD−a
| Cell type and virus | No. (%) of: |
Total | |||
|---|---|---|---|---|---|
| Unenveloped cytoplasmic capsids | Partially enveloped cytoplasmic capsids | Enveloped cytoplasmic particles | Enveloped cell surface particles | ||
| Vero cells | |||||
| F-BAC (w.t.) | 140 (11) | 3 (0.2) | 191 (15) | 939 (74) | 1,273 |
| F-BAC gB−/gD− | 631 (49) | 201 (15) | 271 (21) | 194 (15) | 1,297 |
| F-BAC gD− | 289 (19) | 72 (5) | 325 (21) | 836 (55) | 1,522 |
| F-BAC gB− | 149 (15) | 2 (0.2) | 141 (14) | 714 (71) | 1,006 |
| VD60/gB47 cells | |||||
| F-BAC (w.t.) | 162 (18) | 0 | 90 (10) | 647 (72) | 899 |
| F-BAC gB−/gD− | 225 (20) | 7 (0.6) | 145 (13) | 738 (66) | 1,115 |
a
Vero or VD60/gB47 cells were infected with wild-type (w.t.) F-BAC, F-BAC gB−/gD−, F-BAC gD-kan (F-BAC gD−), or F-BAC gB− for 19 to 28 h and then fixed and processed for electron microscopy. Virus particles were counted in 10 to 20 representative cells.