Table 2.
Numbers of unenveloped, partially enveloped, and fully enveloped particles in F-BAC gB−/gD−-infected HaCaT cellsa
| Virus | No. (%) of: |
Total | |||
|---|---|---|---|---|---|
| Unenveloped cytoplasmic capsids | Partially enveloped cytoplasmic capsids | Enveloped cytoplasmic particles | Enveloped cell surface particles | ||
| F-BAC (w.t.) | 51 (3.2) | 4 (0.25) | 202 (13) | 1,324 (84) | 1,581 |
| F-BAC gB−/gD− | 612 (36) | 234 (14) | 618 (36) | 243 (14) | 1,707 |
| F-BAC gD− | 227 (15) | 62 (4) | 254 (17) | 967 (64) | 1,510 |
| F-BAC gB− | 108 (9) | 2 (0.17) | 139 (11) | 963 (79) | 1,212 |
a
HaCaT cells were infected with wild-type (w.t.) F-BAC, F-BAC gB−/gD−, F-BAC gD-kan (F-BAC gD−), or F-BAC gB− for 19 to 28 h and then fixed and processed for electron microscopy. Virus particles were counted in 10 to 20 representative cells.