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. 2011 May;85(10):5115–5124. doi: 10.1128/JVI.01934-10

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Fig. 5. — Acquisition of MCMV by neonates after 1 day of nursing by latent mothers. One day after birth, C57BL/6 neonates from the mother's own litter (lanes 1 and 2) or age-matched, foster-nursed BALB/c neonates (lanes 3 and 4) were sacrificed. Total RNA from the brain (right panel) and liver (left panel) of individual neonatal mice were collected and analyzed for MCMV IE-1 gene expression by RT-PCR with Southern analysis. In addition, MCMV IE-1 mRNA was detected in lane 1 from brain tissue upon overexposure of the Southern blot. Similar results were obtained in an independent experiment, where expression of MCMV IE-1 mRNA was detected in 2 neonates (1 from the mother's own litter and 1 fostered) nursed by a latent mother.